An Introduction to CryoEM
Learn what types of samples are suitable for cryoEM, what results can be expected, and the steps involved in generating high quality structural data.

Details

When? Tuesday 7 July 2026, 09:30 to 16:30
Where? The University of Leicester, University Road, Leicester
CPD Approved This event is approved for CPD

* This course contributes to the Royal Society of Biology's Technical Skills Certificate

Course location: University of Leicester, England, UK

Overview

Within our cells, biological molecules such as proteins, DNA and RNA are organised into dynamic complexes. Structural biology aims to understand how these complexes assemble and function as molecular machines. Cryo electron microscopy (cryoEM) allows us to study these molecular machines in a frozen hydrated state, preserving them close to their natural environment.

This course provides an introduction to cryoEM and its applications in structural biology. You will learn what types of samples are suitable for cryoEM, what results can be expected, and the steps involved in generating high quality structural data. As part of the training, you will visit and get experience using equipment in the Midlands Regional CryoEM Facility.

The course will focus on single particle analysis (SPA), a computational method that aligns and averages thousands to millions of individual transmission electron microscope (TEM) images. This process produces a 3D cryoEM map with sufficient detail to reveal how proteins function and how they interact with other molecules, including potential drug compounds.

Aims

The course aims to provide participants with an introductory understanding of cryoEM by the following objectives:
  • Gain an understanding of sample preparation and imaging using negative-stain TEM.
  • Obtain experience with vitrification equipment used for cryoEM sample preparation.
  • Get hands-on experience operating an electron microscope for cryoTEM imaging and data collection.
  • Learn the fundamental principles of single-particle analysis, including how 2D projection images are processed and combined to generate a 3D structure.

Who is the course for?

Biological scientists who wish to learn more about cryoEM. Participants should have experience or knowledge of producing purified protein samples (i.e. recombinant protein expression). This course will be useful to those who have knowledge of structural biology without any previous experience of cryoEM or electron microscopy. 

Learning outcomes

Knowledge and understanding
  • Understand which types of biological samples are suitable for analysis by cryoEM. 
  • Appreciate the types of structural and functional information that cryoEM can deliver and learn how to interpret cryoEM results.
  • Learn negative stain TEM.
  • Learn the workflow for cryoEM sample preparation and analysis. 
  • Understand how cryoEM density maps can be used to study protein structure, function, and interactions, including interactions with small-molecule drugs.
Skills, qualities, and attributes
  • Understand suitable cryoEM projects, cryoEM outcomes, and the cryoEM workflow. 

Course tutor

You will be trained by the staff of the Midlands Regional CryoEM Facility; Facility Manager Emma Hesketh, CryoEM Experimental Officer Claudia Lancey, and CryoEM Computational Manager and data‑processing specialist TJ Ragan.

Together, the team routinely train and supervise researchers at every career stage, from Master's students to group leaders, in all aspects of cryoEM. Collectively, they bring over 25 years of experience in cryoEM operations, sample preparation, data acquisition, image processing and analysis.

Technical skills and continuing professional development

This practical based course contributes to the RSB Technical Skills certificate, which can support applications to our professional registers. 

This course is approved for purposes of CPD, and attendees will receive a certificate of attendance and 21 CPD points.

Fees

Members - £600 + 20% VAT
Non-members - £800 + 20% VAT

Get in touch with training@rsb.org.uk to access these rates:

  • Members of Member Organisations, SCAS members - £700 + 20% VAT
  • Non-members who have completed a membership application and made payment - £600 + 20% VAT.

If your organisation wishes to book staff in bulk for this course, please get in touch. Discounts are available for group bookings. 

Contact

For further information about the course please contact Tia Salter, Senior Professional Development Officer at training@rsb.org.uk or on 020 3925 3458.

Special requirements

If you have accessibility requirements, please let us know during your booking, and we will do what we can to accommodate your needs.

Terms and Conditions

By booking to attend this event, you are confirming you have agreed to the RSB's Terms and Conditions which can be found here.

 

Code of Conduct

By its Royal Charter, the Royal Society of Biology (RSB) has the powers to promote, establish and support standards of professional skill and conduct. The RSB is committed to ensuring equal opportunities in the life sciences, and supports diversity throughout the pipeline. We proactively promote a culture of inclusivity within our discipline and the broader STEM community. It is vital at any RSB event or meeting, that everyone attending is free from any form of harassment or discrimination, feels comfortable and safe, and has the opportunity for an enjoyable experience.

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